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$BPF protects vesicle aggregation caused by chemicals. (A) Vesicle aggregation caused by Mg 2+ and PEG6000. The stability of PL liposomes prepared from E. coli phospholipids or INVs was evaluated in the presence of 10 mM MgCl 2 and 3.2% PEG6000. (B) Microscopic observation of liposomes. PL liposomes containing 1% <t>(w/w)</t> <t>NBD-PE</t> were observed by fluorescence microscopy. The lower panel represents a liposome suspension in the HEPES buffer containing 7 mM MgCl 2 . The scale bars represent 10 μm. (C) The effect of Mg 2+ and PEG6000 on the turbidity of vesicles suspension. Absorbance measurements of PL liposomes or INVs suspensions containing the specified concentration of MgCl 2 , with or without 3.2% (w/v) PEG6000, were plotted. Data represent the mean values along with the standard deviations from three independent experiments. (D) BPF activity in the water/methanol phase of the two-phase separated Bligh–Dyer mixture. The BPF activity in the upper water/methanol phase (W) and the lower organic phase (O) of the two-phase separated Bligh–Dyer mixture, obtained from either E. coli cells or INVs, was assessed. The upper water/methanol phase was reconstituted into phospholipid PL liposomes. Liposomes derived from a lower organic phase served as controls. Absorbance of the liposome suspensions was measured in the presence of 7 mM MgCl 2 . Two individual experiments were performed, and the mean values are shown alongside the standard deviation. (E) Urea extraction of BPF from INVs. Urea-washed INVs at the indicated concentration were subjected to resistance assay against 7 mM MgCl 2 or 7 mM MgCl 2 plus 3.2% (w/v) PEG6000. (F) Reconstitution of the urea-extracted fraction into liposomes. The acetone precipitate from the 4 M urea-extracted fraction of INVs was reconstituted into PL liposomes, followed by a resistance assay against 7 mM MgCl 2 . Two individual experiments were performed, and the average values are shown along with the standard deviation.$
Nbd Pe, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 97/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbd+pe/pmc13177006-169-7-13?v=Croda+International+Plc
Average 97 stars, based on 269 article reviews

nbd pe - by Bioz Stars, 2026-07

97/100 stars

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1) Product Images from "Glycolipid MPIase, Essential for Membrane Protein Integration in the Cytoplasmic Membrane of Escherichia coli , Protects Membranes from Aggregation Induced by Chemicals"

Article Title: Glycolipid MPIase, Essential for Membrane Protein Integration in the Cytoplasmic Membrane of Escherichia coli , Protects Membranes from Aggregation Induced by Chemicals

Journal: ACS Omega

doi: 10.1021/acsomega.6c01244

$BPF protects vesicle aggregation caused by chemicals. (A) Vesicle aggregation caused by Mg 2+ and PEG6000. The stability of PL liposomes prepared from E. coli phospholipids or INVs was evaluated in the presence of 10 mM MgCl 2 and 3.2% PEG6000. (B) Microscopic observation of liposomes. PL liposomes containing 1% (w/w) NBD-PE were observed by fluorescence microscopy. The lower panel represents a liposome suspension in the HEPES buffer containing 7 mM MgCl 2 . The scale bars represent 10 μm. (C) The effect of Mg 2+ and PEG6000 on the turbidity of vesicles suspension. Absorbance measurements of PL liposomes or INVs suspensions containing the specified concentration of MgCl 2 , with or without 3.2% (w/v) PEG6000, were plotted. Data represent the mean values along with the standard deviations from three independent experiments. (D) BPF activity in the water/methanol phase of the two-phase separated Bligh–Dyer mixture. The BPF activity in the upper water/methanol phase (W) and the lower organic phase (O) of the two-phase separated Bligh–Dyer mixture, obtained from either E. coli cells or INVs, was assessed. The upper water/methanol phase was reconstituted into phospholipid PL liposomes. Liposomes derived from a lower organic phase served as controls. Absorbance of the liposome suspensions was measured in the presence of 7 mM MgCl 2 . Two individual experiments were performed, and the mean values are shown alongside the standard deviation. (E) Urea extraction of BPF from INVs. Urea-washed INVs at the indicated concentration were subjected to resistance assay against 7 mM MgCl 2 or 7 mM MgCl 2 plus 3.2% (w/v) PEG6000. (F) Reconstitution of the urea-extracted fraction into liposomes. The acetone precipitate from the 4 M urea-extracted fraction of INVs was reconstituted into PL liposomes, followed by a resistance assay against 7 mM MgCl 2 . Two individual experiments were performed, and the average values are shown along with the standard deviation.$
Figure Legend Snippet: BPF protects vesicle aggregation caused by chemicals. (A) Vesicle aggregation caused by Mg 2+ and PEG6000. The stability of PL liposomes prepared from E. coli phospholipids or INVs was evaluated in the presence of 10 mM MgCl 2 and 3.2% PEG6000. (B) Microscopic observation of liposomes. PL liposomes containing 1% (w/w) NBD-PE were observed by fluorescence microscopy. The lower panel represents a liposome suspension in the HEPES buffer containing 7 mM MgCl 2 . The scale bars represent 10 μm. (C) The effect of Mg 2+ and PEG6000 on the turbidity of vesicles suspension. Absorbance measurements of PL liposomes or INVs suspensions containing the specified concentration of MgCl 2 , with or without 3.2% (w/v) PEG6000, were plotted. Data represent the mean values along with the standard deviations from three independent experiments. (D) BPF activity in the water/methanol phase of the two-phase separated Bligh–Dyer mixture. The BPF activity in the upper water/methanol phase (W) and the lower organic phase (O) of the two-phase separated Bligh–Dyer mixture, obtained from either E. coli cells or INVs, was assessed. The upper water/methanol phase was reconstituted into phospholipid PL liposomes. Liposomes derived from a lower organic phase served as controls. Absorbance of the liposome suspensions was measured in the presence of 7 mM MgCl 2 . Two individual experiments were performed, and the mean values are shown alongside the standard deviation. (E) Urea extraction of BPF from INVs. Urea-washed INVs at the indicated concentration were subjected to resistance assay against 7 mM MgCl 2 or 7 mM MgCl 2 plus 3.2% (w/v) PEG6000. (F) Reconstitution of the urea-extracted fraction into liposomes. The acetone precipitate from the 4 M urea-extracted fraction of INVs was reconstituted into PL liposomes, followed by a resistance assay against 7 mM MgCl 2 . Two individual experiments were performed, and the average values are shown along with the standard deviation.

Techniques Used: Liposomes, Fluorescence, Microscopy, Suspension, Concentration Assay, Activity Assay, Derivative Assay, Standard Deviation, Extraction

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Journal: ACS Omega

Article Title: Glycolipid MPIase, Essential for Membrane Protein Integration in the Cytoplasmic Membrane of Escherichia coli , Protects Membranes from Aggregation Induced by Chemicals

doi: 10.1021/acsomega.6c01244

Figure Lengend Snippet: BPF protects vesicle aggregation caused by chemicals. (A) Vesicle aggregation caused by Mg 2+ and PEG6000. The stability of PL liposomes prepared from E. coli phospholipids or INVs was evaluated in the presence of 10 mM MgCl 2 and 3.2% PEG6000. (B) Microscopic observation of liposomes. PL liposomes containing 1% (w/w) NBD-PE were observed by fluorescence microscopy. The lower panel represents a liposome suspension in the HEPES buffer containing 7 mM MgCl 2 . The scale bars represent 10 μm. (C) The effect of Mg 2+ and PEG6000 on the turbidity of vesicles suspension. Absorbance measurements of PL liposomes or INVs suspensions containing the specified concentration of MgCl 2 , with or without 3.2% (w/v) PEG6000, were plotted. Data represent the mean values along with the standard deviations from three independent experiments. (D) BPF activity in the water/methanol phase of the two-phase separated Bligh–Dyer mixture. The BPF activity in the upper water/methanol phase (W) and the lower organic phase (O) of the two-phase separated Bligh–Dyer mixture, obtained from either E. coli cells or INVs, was assessed. The upper water/methanol phase was reconstituted into phospholipid PL liposomes. Liposomes derived from a lower organic phase served as controls. Absorbance of the liposome suspensions was measured in the presence of 7 mM MgCl 2 . Two individual experiments were performed, and the mean values are shown alongside the standard deviation. (E) Urea extraction of BPF from INVs. Urea-washed INVs at the indicated concentration were subjected to resistance assay against 7 mM MgCl 2 or 7 mM MgCl 2 plus 3.2% (w/v) PEG6000. (F) Reconstitution of the urea-extracted fraction into liposomes. The acetone precipitate from the 4 M urea-extracted fraction of INVs was reconstituted into PL liposomes, followed by a resistance assay against 7 mM MgCl 2 . Two individual experiments were performed, and the average values are shown along with the standard deviation.

Article Snippet: E. coli Polar lipid extract and 18:1 NBD-PE, PEG–PE (DOPE-PEG(2000)) were obtained from Avanti Polar Lipids.

Techniques: Liposomes, Fluorescence, Microscopy, Suspension, Concentration Assay, Activity Assay, Derivative Assay, Standard Deviation, Extraction